cloning with restriction enzymes and ligation in one mix - SERP Analysis

https://blog.addgene.org/plasmids-101-restriction-cloning

https://www.neb.com/en-us/tools-and-resources/usage-guidelines/cloning-guide?srsltid=AfmBOoo-EHUhsLY8o3VWUE6feph6yvYQJ_Zf9eJonWM1mXW1b727o99J

https://www.embl.org/groups/protein-expression-purification/services/strategy-and-construct-design/restriction-ligation-cloning/

https://www.snapgene.com/guides/restriction-enzyme-cloning

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-018-0359-7

https://www.researchgate.net/post/Are-you-familiar-with-restriction-digest-with-a-single-enzyme

https://www.reddit.com/r/Biochemistry/comments/1bwfpb9/cloned_plasmid_cannot_be_cut_with_the_same/

https://bitesizebio.com/31136/introduction-restriction-enzyme-cloning/

https://www.researchgate.net/post/Can-you-troubleshoot-my-method-for-cloning-with-NdeI-and-xhoI

https://www.thermofisher.com/us/en/home/life-science/cloning/restriction-enzyme-digestion-and-ligation.html

https://www.reddit.com/r/labrats/comments/1ftdl00/i_cant_figure_out_whats_wrong_with_my_cloning/

https://pmc.ncbi.nlm.nih.gov/articles/PMC2574415/

https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/a/restriction-enzymes-dna-ligase

https://www.genengnews.com/insights/one-step-cloning/

https://www.geneious.com/guides/molecular-cloning-methods

https://www.promegaconnections.com/cloning-tips-for-restriction-enzyme-digested-vectors-and-inserts/

https://www.neb.com/en-nz/new-to-cloning/new-to-cloning?srsltid=AfmBOoqdlM3cCUu-OTL1aY5OuAlmc_Tjm0xoPI_aRNPLjmqACuq2iE57

https://www.epfl.ch/labs/lpbs/internal/general/molecular-cloning-using-restriction-enzymes/

https://www.sciencedirect.com/science/article/pii/S2667237523002084

https://www.neb-online.de/literatur/pdf/Molecular_Cloning_Technical_Guide.pdf

https://virologyj.biomedcentral.com/articles/10.1186/s12985-020-01358-2

https://www.addgene.org/protocols/dna-ligation/

https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/cloning-and-expression/restriction-enzyme-cloning-manual-cloning?srsltid=AfmBOop0GDKwmBJjXBdcRfCAsx6avq_udKNUQYer1f5Uk2RKFZnukavm

https://www.babraham.ac.uk/sites/default/files/2022-07/Cloning.doc

https://www.thermofisher.com/us/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/cloning/traditional-cloning-basics.html

https://www.sciencedirect.com/topics/immunology-and-microbiology/cloning-enzyme

https://www.youtube.com/watch?v=MXMR1i4qwkM

https://bitesizebio.com/23458/protocols-for-cloning-without-restriction-enzymes-or-ligases/

https://www.neb-online.de/wp-content/uploads/2018/05/MolCloning_Tech_Guide_D-F_1805_LowRes.pdf

https://www.jenabioscience.com/molecular-biology/cloning-and-mutagenesis/ligase-free-cloning/pcr-237-pfu-x-core-kit

https://socratic.org/questions/how-do-you-choose-which-restriction-enzymes-to-use-when-cloning-a-gene

https://www.promega.com/resources/guides/nucleic-acid-analysis/subcloning/

https://wolfson.huji.ac.il/expression/cloning_res_enz.html

https://www.quora.com/Plasmid-Cloning-Can-you-use-restriction-enzymes-from-different-companies-in-the-same-digest

https://academic.oup.com/nar/article/27/19/e26/1059172

https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpmb.115

https://mcmanuslab.ucsf.edu/protocol/cloning-restriction-digest

https://www.labxchange.org/library/items/lb:LabXchange:3e1b79de:html:1

https://www.tandfonline.com/doi/full/10.2144/000114324

https://mendelgen.com/restriction-enzyme-cloning

https://www.youtube.com/watch?v=j4aSfi8MaXA

https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/overview-dna-cloning

https://www.takarabio.com/learning-centers/cloning/in-fusion-cloning-faqs?srsltid=AfmBOooSRIMC85bn4djOWy7xufFbjhvJEJS7BKT1iMO0XmjKdJF1CMyC

https://www.nature.com/articles/s41598-024-77327-4

https://journals.plos.org/plosone/article/figures?id=10.1371/journal.pone.0107907

https://www.cell.com/cell-reports-methods/pdf/S2667-2375(23)00208-4.pdf

https://www.qiagen.com/us/applications/enzymes/cloning-and-dna-recombination

https://www.bio-rad.com/webroot/web/pdf/lse/literature/10011370A.pdf

https://www.biocat.com/bc/files/Gibson_Guide_V2_101417_web_version_8.5_x_11_FINAL.pdf

https://www.snapgene.com/guides/the-plasmid-cloning-cycle

https://biology.stackexchange.com/questions/2651/open-protocol-for-ligase-independent-cloning

https://barricklab.org/twiki/bin/view/Lab/GoldenGateAssembly

https://pressbooks.bccampus.ca/kathleef/chapter/chapter-6-plasmids-and-cloning-basics/

https://pipebio.com/blog/cloning

https://www.protocol-online.org/biology-forums-2/posts/11166.html

https://www.idtdna.com/pages/education/decoded/article/cloning-strategies-part-3-blunt-end-cloning

https://www.weizmann.ac.il/Biomolecular_Sciences/Bibi/sites/Biomolecular_Sciences.Bibi/files/uploads/RF%20cloning.pdf

https://static.igem.org/mediawiki/2019/1/1c/T--Technion-Israel--protocols-cloning_using_multiple_enzymes.pdf

https://goldbio.com/articles/article/Restriction-Enzyme-Cloning-Troubleshooting?srsltid=AfmBOoqLYLQpP6OL3I5X5uuimLSIgLD-LJjadimAFrD2sTTnZU9PbF2P

https://www.genscript.com/gsfiles/techfiles/Cloning-Guide-screen-reading-file.pdf

https://journals.asm.org/doi/10.1128/AEM.00844-12

https://academic.oup.com/nar/article/25/20/4165/1027598

https://www.mdpi.com/1467-3045/47/1/17

https://www.arcticzymes.com/application/dephosphorylation-prior-to-cloning

https://coleman-lab.org/wp-content/uploads/2021/04/Cloning-into-plasmids-digestion-ligation-troubleshooting-.pdf

https://qb3.berkeley.edu/education/lab-fundamentals-bootcamp/manual/cloning/gibson-assembly/

https://www.protocols.io/view/around-the-horn-pcr-and-cloning-rf2d3qe

https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/v/dna-cloning-and-recombinant-dna

https://sharebiology.com/infusion-cloning/

https://manuals.cellecta.com/cdna-cloning-and-expression-vector-user-manual/v1/en/topic/cdna-cloning

https://www.takarabio.com/learning-centers/cloning/in-fusion-cloning-general-information/in-fusion-cloning-and-competition/choosing-a-seamless-cloning-method?srsltid=AfmBOookVs1CtXI2VknF9m0vtWwdgXxEZAMkUbNU5UzsCogwEwOedYRK

https://ocw.mit.edu/courses/7-15-experimental-molecular-genetics-spring-2015/857fcd5fb6b6b392ab478e8167337b8f_MIT7_15S15_Molecular.pdf

https://www.youtube.com/watch?v=ekmdt7b3vb0

https://en.wikipedia.org/wiki/Golden_Gate_Cloning

https://blog.quartzy.com/2017/02/14/molecular-cloning-pcr-products-restriction-digestion

https://www.promega.com/-/media/files/resources/protocols/technical-manuals/0/pgem-t-and-pgem-t-easy-vector-systems-protocol.pdf

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0005553

https://bmb.natsci.msu.edu/sites/_bmb/assets/File/Sharkey_lab/Restriction%20Digest%2C%20Dephosphorylation%2C%20Gel%20Purification%2C%20Ligation.pdf

https://static.igem.org/mediawiki/2021/8/88/T--UParis_BME--cloning_RE.pdf

https://onlinelibrary.wiley.com/doi/10.1111/gtc.13034

https://www.abmgood.com/pro-ligation-free-cloning-kit-e086.html

https://barricklab.org/twiki/bin/view/Lab/ProtocolsRestrictionEnzymeCloning

https://www.nature.com/articles/s41598-019-42868-6

https://www.youtube.com/watch?v=oJYrIT8tTso

https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/cloning-and-expression/dna-ligation-kit?srsltid=AfmBOopZlKe2ftnxK6XFsBvqRZBi7sjDVxHllaolpnGvvTqFf35RnRsQ

https://www.tandfonline.com/doi/pdf/10.2144/99276rr02

https://mendelgen.com/ta-cloning

https://www.promegaconnections.com/pgem-ligation/

https://www.youtube.com/watch?v=nfTVj85dX9U&pp=0gcJCdgAo7VqN5tD

https://www.toyobo-global.com/products/lifescience/products/cloning.html

https://www.geneious.com/tutorials/restriction-cloning

https://help.benchling.com/hc/en-us/articles/9684255457805-Molecular-Cloning-Methods

https://ncert.nic.in/textbook/pdf/lebo109.pdf

https://www.genecopoeia.com/product/cloning_products/ezshuttle-recombination-cloning-system/

https://www.youtube.com/watch?v=sOjmMSHexW0

https://www.systembio.com/products/gene-expression-systems/cold-fusion-cloning/cold-fusion-cloning-kit-with-competent-cells

https://www.nippongenetics.eu/en/product-category/cloning/

https://portlandpress.com/bioscirep/article/37/2/BSR20160608/83272/Mega-primer-mediated-molecular-cloning-strategy

https://pipettejockey.com/2019/11/22/teda-cloning-cheap-easy-gibson-alternative/